Thermo-responsive non-woven scaffolds for “smart” 3D cell culture
نویسندگان
چکیده
منابع مشابه
Thermo-responsive non-woven scaffolds for "smart" 3D cell culture.
The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (PET), and nylon that had been grafted with PNIPAAm were tested for their abili...
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There are numerous approaches for producing natural and synthetic 3D scaffolds that support the proliferation of mammalian cells. 3D scaffolds better represent the natural cellular microenvironment and have many potential applications in vitro and in vivo. Here, we demonstrate that 3D cellulose scaffolds produced by decellularizing apple hypanthium tissue can be employed for in vitro 3D culture...
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The biggest challenge of designing and implementing an in vitro study is developing a microenvironment that most closely represents the interactions observed in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM ) proteins necessary ...
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Stem cells are found in naturally occurring 3D microenvironments in vivo, which are often referred to as the stem cell niche. Culturing stem cells inside of 3D biomaterial scaffolds provides a way to accurately mimic these microenvironments, providing an advantage over traditional 2D culture methods using polystyrene as well as a method for engineering replacement tissues. While 2D tissue cultu...
متن کاملA THERMO-RESPONSIVE PNIPAAm-GRAFTED-PDMS SURFACE USED FOR CELL CULTURE IN MICROFLUIDIC CHANNELS
A thermo-responsive PNIPAAm-grafted-PDMS (PNIPAAm-g-PDMS) surface was used for cell culture. Cos7 cells could adhere to the PNIPAAm-g-PDMS surface and proliferated therein at 37 C. The cultured cells could detach from the surface at room temperature without trypsin digestion. The viability of cells harvested from the PNIPAAm-g-PDMS surface via lowering temperature was higher than that of cells ...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2012
ISSN: 0006-3592
DOI: 10.1002/bit.24469